Tips for Successful RNA-seq Experiments

Published Di Zheng on Fri, 06/02/2023 - 11:25

Every Monday from 1pm – 2pm notable cancer researchers, clinicians, nurses and other experts from around the globe share their insights, research or news on a broad range of topics at the VCCC Alliance Monday Lunch Live forum.

The following area contains all our recordings from 2020. 

Tips for Successful Single Cell and Single Nucleus RNA-seq Experiments.

Monday Lunch Livestream

With Dr Luciano Martelotto

10 August 2020

Hear from newly appointed Scientific Director of the Single Cell Core at the Laboratory of Harvard Medical School, who describes how to design a single cell genomics experiment.

Single cell and single nucleus genomics offer the amazing opportunity to enhance the resolution of our understanding of biology. However, successful experimentation is only possible when every step is done properly.

Dr Luciano Martelotto’s presentation covers critical factors needed to be considered from sample accrual and storage, to sample processing and sequencing. He describes the best practices of single cell genomics that researchers can take back to their labs. He also provides practical tips on what to do and what not to do.

His presentation may interest those with and without expertise in the field and for those who want to reinforce or learn how to design a single cell genomics experiment.

Dr Luciano Martelotto

Dr Luciano Martelotto has been recently appointed Scientific Director of the Single Cell Core Laboratory of Harvard Medical School (HMS), Department of Systems Biology. Prior to joining HMS, he was the Head of the Single Cell Innovation Laboratory at The University of Melbourne Centre for Cancer Research within the Victoria Comprehensive Cancer Centre and as such has spearheaded the development and single cell-based genomics.

Amongst his ground-breaking achievements is the establishment of a single-cell analysis of fresh, frozen and archival human samples, which facilitated our understanding of intra-tumour genetic heterogeneity (Xue, Martelotto et al.Nature Medicine 2017; Martelotto et al.Nature Medicine 2017).

His current team develops, implements and offers methods of single cell genomics in order to build a bridge between researchers and single-cell technologies in their quest to understand how cells collectively perform systems-level functions in healthy and diseased states. Through collaborative efforts with leading Institutes in single cell genomics and spatial transcriptomics such as the Peter MacCallum Cancer Centre, The University of Melbourne Centre for Cancer Research, The Garvan Institute of Medical Research and the Memorial Sloan Kettering Cancer Center as well as genomics companies like 10x Genomics and Illumina his team addresses how cellular heterogeneity shapes complex biological systems.

Course Details

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Course type
60 mins
Curriculum Area
Research (incl. Clinical Trials)
Clinical Care
Early to mid career researcher
Senior researcher / scientist
Monday Lunch Live

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